Journal: Cell reports

Article Title: Chronic Corticosterone Elevation Suppresses Adult Hippocampal Neurogenesis by Hyperphosphorylating Huntingtin.

doi: 10.1016/j.celrep.2020.107865

Figure Lengend Snippet: Figure 4. Phosphorylation of HTT at S1181/S1201 Influences Hippocampal Neurogenesis (A–C) Cells counts were performed in the DG of vehicle- and CORT-treated WT and HdhS1181A/S1201A mice (at least 4 for animals per condition) to quantify proliferation (Ki67+ cells, A), survival (BrdU+ cells, B), and immature neurons (DCX+ cells, C). *p < 0.05, **p < 0.01, ***p < 0.001. (D) Representative images show BrdU+ surviving cells (cyan) expressing (or not) the terminal marker for granule cell differentiation calbindin (magenta) on DG sections of WT and HdhS1181A/S1201A mice treated with vehicle or CORT. (E) The histogram shows the quantification of the number of BrdU+ and calbindin+ cells (3 animals per condition). *p <0.05, **p < 0.01. (F and G) Dendritic length (F) and complexity (G) were evaluated in both genotypes and treatment conditions (3 animals per condition). (H) Representative drawings of dendrites of DCX-positive neurons. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 comparing the HdhS1181A/S1201A vehicle group with the WT vehicle group, $p < 0.05, $$p < 0.01, $$$p < 0.001, $$$$p < 0.0001 comparing the HdhS1181A/S1201A -CORT group with the WT CORT group. Error bars, SEM. See also Figure S2.

Article Snippet: Slices were rinsed and blocked in 3% normal donkey serum in PBS containing 0.3% Triton X-100 for 1h at room temperature then incubated overnight at room temperature in 1:100 of mouse anti-BrdU antibody (#347580, BDBiosciences) and 1:500 rabbit anti-calbindin antibody (#13176, Cell Signaling Technologies).

Techniques: Phospho-proteomics, Expressing, Marker, Cell Differentiation

Journal: Cell reports

Article Title: Overlapping Activities of Two Neuronal Splicing Factors Switch the GABA Effect from Excitatory to Inhibitory by Regulating REST

doi: 10.1016/j.celrep.2019.03.072

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse anti-calbindin D-28K monoclonal antibody (clone CB-955) , Acris Antibodies , Cat# AM08219SU-N; RRID: AB_1954252.

Techniques: Expressing, Plasmid Preparation, Recombinant, SYBR Green Assay, Reporter Assay, Isolation, Staining, Microarray, Clone Assay, Software

Journal: Frontiers in Neuroscience

Article Title: Dystrophin Is Required for the Proper Timing in Retinal Histogenesis: A Thorough Investigation on the mdx Mouse Model of Duchenne Muscular Dystrophy

doi: 10.3389/fnins.2020.00760

Figure Lengend Snippet: Primary antibodies used for immunofluorescence.

Article Snippet: Rabbit anti-calbindin (HC) , 1:1000 , Merck Millipore (Billerica, MA, United States).

Techniques: Immunofluorescence